过表达FGF10促进山羊皮下前体脂肪细胞分化

doi:10.11843/j.issn.0366-6964.2019.10.003

Abstract

Abstract: The aim of this study was to explore the effect and mechanism of FGF10 overexpression on the differentiation of subcutaneous preadipocytes in goats, which was mediated by adenovirus transporters. In this study, the subcutaneous preadipocytes were separated from goat by collagenase digestion, pAdTrack-CMV-FGF10 was constructed successfully through AdEasy adenovirus packaging system, and then was used to infect cells. In morphology, Oil red O staining was used for investigating the effect of FGF10 on adipogenic differentiation. The mRNA levels of adipocyte differentiation marker genes, lipid metabolism related genes, fibroblast growth factor receptors (FGFRs) and Kruppel like factors (KLFs) were measured by quantitative real-time PCR (qPCR). The results showed that the lipid droplets accumulation increased after 2 days of FGF10 overexpression, compared with control group.The mRNA levels of C/EBPα, LPL, ACACA, FGFR1 and FGFR3 significantly increased(P<0.01). The relative expression of FASN and ATGL were significantly up-and down-regulated (P<0.05), respectively. Meanwhile, overexpression of FGF10 significantly up-regulated the mRNA expression of KLF8-10, 16 and 17 genes(P<0.05), while significantly down-regulated the mRNA expression of KLF1, 2, 4 and 15 genes(P<0.05). These results indicate that FGF10 overexpression promotes lipid droplets accumulate possibly by regulating the expression of C/EBPα, LPL, FASN, ACACA, ATGL and partial members of KLFs. This study provides an important data support for elucidating the molecular mechanism for FGF10 controlling fat deposition in different parts of goats.

CLC Number:

S827.2

XU Qing, LI Qian, WANG Yong, ZHU Jiangjiang, ZHANG Ya'nan, LIN Yaqiu. Overexpression of FGF10 Promotes the Differentiation of Subcutaneous Preadipocyte in Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 1972-1984.

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Overexpression of FGF10 Promotes the Differentiation of Subcutaneous Preadipocyte in Goat

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